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. 2019 Jun 18;20(12):2978. doi: 10.3390/ijms20122978

Figure 2.

Figure 2

Subcellular localizations of PlCYP5 and PlCYP7 in Nicotiana benthamiana. Agrobacterium tumefaciens was used to transiently express eGFP::PlCYP5 or eGFP::PlCYP7 fusion proteins in the leaves of N. benthamiana, and transient expression of eGFP was used as the control. Subcellular localizations of the fused proteins were visualized using fluorescence microscopy at 48 h after infiltration, and 4’, 6-diamidino-2-phenylindole was used to stain the nucleus of epidermal cells before microscopic observation. The arrows indicate proteins localized in the nucleus.