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. 2019 Jun 18;20(12):2978. doi: 10.3390/ijms20122978

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Assay of PlCYPs’ response to different abiotic stresses. (A) Growth analysis of E. coli cells containing an empty vector (pET-28a) and recombinant vectors (pET-PlCYP4 and pET-PlCYP6) was carried on Luria-Bertani (LB) liquid medium with two treatments (supplemented with 1 mM H₂O₂ and 70 °C for 20 min). The treated cultures were diluted to a suitable concentration and then spread onto the LB plate to count the number of living bacteria. (B) Statistical analysis of the bacterial colonies. The error bar represents the SD of three replicates. * denotes p < 0.01.