Figure 2.

CTCs express tissue-specific markers and were obtained from mice that developed cancer metastasis. (A,C) Immunofluorescence staining for CREB3L3, a liver-specific marker; and (B,D) western blotting for CREB3L3. DAPI nuclear staining is shown in blue; CREB3L3 cytoplasmic staining is shown in red. (E) H&E staining of tumor and lung tissues from implantation of HCC cell line into BALB/c mice demonstrates evidence of cancer metastasis to lungs of cancer-bearing mice. (F) Western blotting was carried out to demonstrate prostate cancer origin for both CRPC primary tumor-derived and CTC lines. Experiments were carried out at least three times.