Figure 4.

Expression profiles of APX, GPX, CAT and SOD genes upon desiccation stress in the resurrection species. The RNA-seq data from (A) Boea hygrometrica, (B) Selaginella lepidophylla, (C) Xerophyta viscosa and (D) Oropetium thomaeum were analyzed to obtain normalized expression values (TMM) for APX, GPX, CAT and SOD genes. Color scale represents the log₂ transformed and mean centered TMM values. Genes with low or no expression values were removed. HD: fully hydrated; RWC: relative water content; 0h: three years after desiccation; REH: hours after rehydration; DEH: hours after dehydration post-rehydration; TWC: turgid water content; FL: fresh leaves; DL: desiccated leaves; RL: rehydrated leaves. RWC is calculated as (fresh weight–dry weight) × 100/(turgid weight – dry weight). TWC is the ratio of (fresh weight–dry weight) to dry weight.