Figure 6.

Preferential adhesion of melanoma cells (NZM7) to hCMVEC endothelial junctions. NZM7 cells were live-stained with CMFDA (green) and applied to the apical face of hCMVECs. Cells were co-cultured for 30 min, fixed and endothelial junctions were visualized with anti-α-catenin (red). Nuclei were visualized using DAPI. (A–F) Multiple examples of a melanoma cell (green/yellow) localising at endothelial junctions (red). Images (A) and (D) are good examples of the melanoma cell residing at a multi-junctional location (corner of several endothelial cells). (B) shows a small melanoma cells at the junction of a large clearly visible endothelial cell. (C) is an example of multiple melanoma cells adhered at the same junctional positon. (E) exemplifies a large melanoma cell positioned at multiple endothelial junctions and (F) shows a melanoma cells on the junctional border of four neighbouring endothelial cells. Images shown are representative of two independent experiments. Scale bar is 20 µm.