Table 3.

Advantages and disadvantages of BiFC and BiLC techniques.

BiFC
Advantages	Disadvantages
Straightforward technique	Need for tagged proteins (GPCRs)
High-throughput experiments	Autofluorescence
Imaging microscopy: localization of the interaction	Photobleaching
Study intact cells	Measuring dynamics: limited (maturation time) Not applicable for studying inhibition of interactions
BiLC
Advantages	Disadvantages
Straightforward technique	Need for tagged proteins (GPCRs)
High-throughput experiments	Requires a substrate
Kinetic measurements	Detection of localization: limited
Study intact cells
In vivo application