Table 5.

An overview of GPCR–GPCR interactions, shown by fluorescence complementation assays.

GPCR Dimer	Oligomeric Type	PCA	Split Biosensor	Fragments	Negative Control	Cell-Type	Year	Ref.	In Vivo or Native Tissue Evidence	Ref.
mGluR5− D2R	Heterodimer	BiFC	YFP	1–155, 155–231	GABAB2	HEK	2009	[130]	Yes	[130]
D2R − D2R	Homodimer	BiFC	YFP	1–155, 156–238	D1R	HEK	2015	[118]	Yes	[131,132]
A2A − D2LR	Heterodimer	MBiFC	Venus/Cerulean	1–172, 155–238	D1	CAD	2008	[59]	Yes	[130,133,134,135,136,137]
D2LR − CB1	Heterodimer	MBiFC	Venus/Cerulean	1–172, 155–238	M4	CAD	2010	[62]	Yes	[133]
D2LR − D2LR	Oligomer	MBiFC	Venus/Cerulean	1–172, 155–238	-	CAD	2010	[62]	-	-
D2SR − D2SR	Homodimer	BiFC	Venus	1–155, 156–240	CD8	HEK293T	2008	[100]	Yes	[131,132]
AT1 − AT2	Homo- and heterodimer	BiFC	Venus	1–158,159–239	ATIP	HEK293FT	2011	[125]	Yes	[138,139,140]
CXCR4 − CXCR4	Homodimer	BiFC	vYFP	1–465, 466–720	D2R	HEK293	2014	[99]	-	-
A2A − A2A	Homodimer	MBiFC	Venus/Cerulean	1–172, 155–238	-	CAD	2008	[59]	-	-
A2A − A2A	Homodimer	BiFC	YFP	1–155, 155–238	Non-fused A1 (competition)	HEK293T	2018	[65]	-	-
A2A − A1	Heterodimer	BiFC	YFP	1–155, 155–238	-	HEK293T	2018	[65]	Yes	[141]
GHSR1a-OX1R	Heterodimer	BiFC	Venus	1–172, 156–239	-	HEK293T	2018	[64]	-	-
β2AR − β2AR	Oligomer	BiFC	− 15sfGFP		-	HeLa	2019	[67]	-	-
A2A − A2A	Homodimer	BiFC	YFP	1–155,156–239	GABAB2	CHO	2018	[66]	-	-
A2B − A2A	Heterodimer	BiFC	YFP	1–155,156–239	GABAB2	CHO	2018	[66]	-	-
FFAR3 − FFAR3	Homodimer	BiFC	Venus	1–155 (I152L), 155–239	P2RY1	HEK293T	2018	[63]	-	-
FFAR2 − FFAR3	Heterodimer	BiFC	Venus	1–155 (I152L), 155–238	P2RY1	HEK293T	2018	[63]	-	-
mGluR2 − mGluR2	Homodimer	BiFC	mCitrine	1–172, 155–238	-	HEK293T	2016	[142]	-	-
α1b − α1b	Homodimer	BiFC	eYFP	1–172, 155–238	-	HEK293T	2007	[143]	-	-