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Pre-formed droplets are used to stimulate and extract analyte from a substrate, such as cell culture. (a) schematic of a device in operation; (b) an opening in the chip allows for droplet to merge with the hydrophilic substrate; (c) time-lapse bright-field images (side view) of the droplet extracting contents at 0 ms, 30 ms, 60 ms and 90 ms. (Reprinted with permission from Reference [10]. Copyright 2008 National Academy of Sciences, USA).
