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. 2019 May 31;11(6):763. doi: 10.3390/cancers11060763

Figure 5.

Figure 5

PC-3 cell injection at different times during heterotopic humanized bone/marrow organ development. About four weeks from of hBMSCs transplantation, extraskeletal ossicles present abundant bone and fibrous tissue, bone but not marrow (a). CD146+ cells are mixed with fibrous cells and are in contact with vessels (b). About eight weeks from transplantation, a complete marrow microenvironment has been recreated in extraskeletal ossicle and CD146+ can be detected as pericytes around BM sinusoids (c,d). Injection of PC3 cells four weeks after hBMSCs transplantation resulted in unsuccessful migration/survival of human cancer cells in heterotopic ossicles (e,f). PC3 cells injected eight weeks from hBMSCs transplants were able to grow in ectopic humanized BM ossicles. Immunolabelling for human CD146 identified both metastatic foci of PC3 cells (h) and the human advential reticular cells wrapped around the sinusoid wall (g). H&E, Hematoxylin/Eosin; ft, fibrous tissue; s, sinusoid; hem, hematopoiesis, HA, hydroxyapatite. He, Hematoxylin counterstaining. Scale bar = 30, 40, 50, 80, 100 μm.