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. 2019 Jun 6;17(6):340. doi: 10.3390/md17060340

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Planarians as neoclassical models in neuropharmacology and predictive neurotoxicology. (A) Regeneration of planarian CNS as well as straightforward applications of in vivo RNA interference in high throughput can be used to study of neuroregeneration pathways and pharmacological manipulations in brain injury. (B) Visualization of CNS regeneration using immunohistochemical detection of DjSYT protein (shown in green) in control and in Readyknock animals 7 days after decapitation. (C) Neurobehavioural analysis utilizing chemotactic behaviour of planarians in chemoattractant concentration gradient field. (D) Neurobehavioural analysis utilizing thigmotactic behaviour of planarians in structured environment. Reprinted and adapted from [104].