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. 2019 Apr 27;8(2):25. doi: 10.3390/biology8020025

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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EtOH treatment reduced BV-2 cell viability in a dose-dependent manner. BV-2 cells were treated with 50 and 100 mM of EtOH at 72 h and cell viability was tested. Cells were washed and labeled with Annexin V-FITC and PI to discriminate apoptotic and healthy cells. For this experiment, live cells (E3: 84.67%) were gated to determine the percentage of early (Q3), late (Q2) and apoptotic (Q1) cells after EtOH exposure. Early apoptotic (Q3) and apoptotic (Q1) cell were higher when dosed with 100 mM; however, 50 mM showed higher a percentage of late apoptotic (Q2) cells. FSC: Forward Scatter; SSC: Side Scatter.