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Expression of exosome biogenesis and secretory proteins. (A) Cell lysates (at 72 h EtOH administration) and (B,C) exosome proteins (at 48 and 72 h EtOH administration) were evaluated for Rab 7 expression. In order to obtain quantitative results, cell lysates were subjected to dot blot analysis and exosomes were subjected to ELISA. Experiments involving dot blot analysis and ELISA were performed with four to six independent replicates.
