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. 2019 Jun 6;8(6):547. doi: 10.3390/cells8060547

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Glo1 depletion and related downstream events are under the partial control of IL-1β in CAL62 cells. Effects of IL-1β on (A) Glo1 enzyme activity; (B) Glo1 transcript and protein levels; (C) intracellular levels of MG-H1; (D) TGF-β1 and p-FAK levels, measured in the culture supernatant or lysate of CAL62, respectively; (E) migration and invasion capabilities; and (F) IL-1β signaling, evaluated by the levels of IL1 receptor type I (ILR1) (measured in the cell lysates), phospho-TAK1 (p-TAK1) (measured in the cell lysates), and P65 NF-kB (measured in the nuclear extracts). Western blots are representative of three different cultures, each tested in triplicate. β-actin was used as internal loading control for WB normalization. Histograms indicate mean ± SD of three different cultures, each tested in triplicate. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to untreated cells.