Figure 11.

Resveratrol affects the CAL62 cell aggressive phenotype by reducing migration and invasion through the inhibition of IL-1β-driven, Glo1/MG-H1-mediated TGF-β1/FAK signaling. Effect of resveratrol on (A) IL-1β levels, evaluated by an ELISA kit in cell culture medium; (B) Glo1-specific activity, measured by spectrophotometry; and (C) migration and invasion capabilities, evaluated by specific assays. Effect of IL-1β, under resveratrol administration, on (D) Glo1 enzyme activity and (E) migration and invasion capabilities. Histograms indicate mean ± SD of three different cultures, and each was tested in triplicate. * p < 0.05, ** p < 0.01, and *** p < 0.001.