Figure 1.

Cloning of LlaCIPK gene:LlaCIPK RACE PCR products of GeneRacer™ primer and gene specific primers. (A,B) Electrophoretic analysis of LlaCIPK 5′ RACE PCR products & 3′ RACE PCR products respectively. Lane M-DNA Marker & 1, 2, 3 are RACE amplified fragments. (C) Autoradiogram of Southern blot of LlaCIPK. Genomic DNA (20 µg) was digested with four different restriction enzymes and hybridized with LlaCIPK gene probe labeled with (α⁻³²P) dCTP by random priming method.