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. 2019 Jun 12;10(6):446. doi: 10.3390/genes10060446

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of LlaCIPK expression profiles in Lepidium seedlings subjected to different treatments: (A) The Lepidium seedlings were subjected to cold stress (4 °C) for the indicated times (0, 3, 6, 12 and 24 h), (B) The seedlings were sprayed with different concentrations of SA and ABA solution. (C) The seedlings were sprayed with different concentrations of Ethylene and CaCl₂ solution. The expression level of LlaCIPK at 0 h or in mock treatments was used as control (calibrator). Relative transcript abundance was calculated using the comparative 2^-ΔΔCT (cycle threshold) method. Error bars represent standard error of means based on three independent biological replicates.