Figure 3.

Effect of dihydrocapsaicin on EGF-induced c-Fos, c-Jun, and AP-1 activities and COX-2 promoter activity. (A, B) TransAM™ AP-1 family transcription assay kit was used to assess c-Fos and c-Jun activity. Nuclear protein extracts from JB6 P+ cells were used after the indicated treatment. Data are presented as means ± S.D. (C) JB6 P+ cells stably expressing an AP-1 luciferase reporter plasmid were treated as indicated. (D) JB6 P+ cells stably expressing COX-2 promoter reporter plasmid were treated as indicated. ** p < 0.01, significant differences between the group treated with EGF alone and co-treated with dihydrocapsaicin and EGF.