Figure 6.

Hrq1 and Pif1ΔN are still able to interact to regulate telomerase activity. (A) In vitro telomerase extension of the Tel50 substrate in the absence or presence of the indicated concentration of both recombinant Hrq1 and Pif1ΔN (e.g., 45 nM indicates that 45 nM of each helicase was added). The direct extensions (Type I; T51–T58) and nuclease-cleaved extension products (T26–T32) [14] are noted on the left. The positions of the Tel30 and Tel50 markers are shown on the right. (B) Quantification of the telomerase activity shown in (A); mean values and standard deviation are shown. Total activity refers to the total amount of signal in the lanes. Significant differences were determined by multiple t tests using the Holm–Sidak method, with α = 5% and without assuming a consistent SD (n = 3). *, p < 0.01 and **, p < 0.001; all comparisons were made against the reactions lacking added helicase.