Table 1.
Plasmids used in this study.
| Name | Description |
|---|---|
| pRS414 | TRP1, ARS1, CEN4 empty vector [15] |
| pMB282 | Pif1-3xFLAG cloned into pRS414 under the control of the RRM3 regulatory sequences [16] |
| pMB327 | Pif1ΔN-3xFLAG cloned into pRS414 under the control of the RRM3 regulatory sequences [16] |
| pESC-URA | Multi-copy vector enabling epitope tagging of genes cloned under the control of the bidirectional GAL1,10 promoter |
| pUC19(+)TEL | pUC19 harboring yeast telomeric repeat sequence DNA cloned into the EcoRI site [17] |
| pMB526 | Pif1 cloned into pESC-URA, enabling galactose induction and epitope-tagged with FLAG |
| pMB540 | Pif1ΔN cloned into pESC-URA, enabling galactose induction and epitope-tagged with FLAG |
| pSUMO-Pif1 | Nuclear isoform of Pif1 cloned into the pSUMO vector |
| pSUMO-Pif1ΔN | Pif1ΔN cloned into the pSUMO vector |