pRS414 |
TRP1, ARS1, CEN4 empty vector [15] |
pMB282 |
Pif1-3xFLAG cloned into pRS414 under the control of the RRM3 regulatory sequences [16] |
pMB327 |
Pif1ΔN-3xFLAG cloned into pRS414 under the control of the RRM3 regulatory sequences [16] |
pESC-URA |
Multi-copy vector enabling epitope tagging of genes cloned under the control of the bidirectional GAL1,10 promoter |
pUC19(+)TEL |
pUC19 harboring yeast telomeric repeat sequence DNA cloned into the EcoRI site [17] |
pMB526 |
Pif1 cloned into pESC-URA, enabling galactose induction and epitope-tagged with FLAG |
pMB540 |
Pif1ΔN cloned into pESC-URA, enabling galactose induction and epitope-tagged with FLAG |
pSUMO-Pif1 |
Nuclear isoform of Pif1 cloned into the pSUMO vector |
pSUMO-Pif1ΔN |
Pif1ΔN cloned into the pSUMO vector |