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. 2019 Jun 14;20(12):2902. doi: 10.3390/ijms20122902

Figure 3.

Figure 3

Effect of butyrate on PDK1 protein and phosphorylation level in VSMCs. Proliferating VSMCs were treated with (BA) or without (Con) butyrate for indicated periods of time and then cell lysates were prepared. Cell lysates were processed for PDK1 Western blotting to assess the effect of butyrate-inhibited PI3K activity on PDK1 protein and activation levels by measuring total and phosphorylated PDK1 levels, respectively. The data obtained were analyzed and presented as mean ± S.D. (A) PDK1 protein level measured by using antibody specific to total PDK1 (B) Phosphorylation of PDK1 assessed by using antibody specific to Ser241-phosphorylated PDK1 indicated significant reduction in activation of PDK1 in VSMCs treated with butyrate for 24 h and 30 h (* p < 0.01 vs 24 h control (Con), ** p < 0.001 vs 30 h control (Con).