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. 2019 Jun 14;20(12):2902. doi: 10.3390/ijms20122902

Figure 4.

Figure 4

Inhibition of Akt activation by butyrate. VSMCs were treated with (BA) or without (Con) butyrate for indicated periods of time and then processed for western analysis of total Akt, Thr308 and Ser473 phosphorylated Akt as described in Methods Section. Total Akt levels were determined by immunoblotting with Akt-specific antibody and used as normalizing controls. Respective data are presented as mean ± S.D. (A) Levels of Thr308-phosphorylated Akt were determined by immunoblotting with antibody specific to phospho-Thr308Akt. (B) Levels of Ser473-phosphorylated Akt were assessed by immunoblotting with antibody specific to phospho-Ser473Akt. ℗℗ p < 0.001 vs 6 h control (Con); ** p< 0.001 vs 6 h control (Con), * p < 0.01 vs 24 h control (Con).