Figure 8.

Inhibition of activation of mTOR and its downstream targets by butyrate in VSMCs. Cell lysates prepared from VSMCs treated with (BA) or without (Con) butyrate were analyzed by Western blotting using antibodies specific to (A) phosphorylated mTOR, (B) Phosphorylated p70S6 kinase, (C) phosphorylated S6 ribosomal protein and (D) phosphorylated 4E-BP1. Appropriate second antibodies labelled with IRDye 680LT or IRDye 800CW were used for detecting target proteins. LI-COR CLx imaging system and Image Studio software were used for scanning and quantitating signal intensities. (* p < 0.01 vs Control).