Comparison between BW (A), food (g/day) (B), and water (mL/day) consumption (C) (mean ± sd) of animals fed with NEAA-100% and EAA-30% diets, StD and EAA-100% diet after 6 weeks. NEAA-100% and EAA-30% diets drive rapid BW decrease, whereas EAA-100% diet slowly increases BW compared to StD (A). Note that EAA-100% diet was consumed in the same amount as NEAA-100% and EAA-30% diets (B). NEAA-100%-fed animals showed a higher water intake compared to StD, whereas EAA-30% and EAA-100%-fed animals had a lower water consumption than StD (C). Black square, EAA-100%; gray rhombus, NEAA-100%; gray triangle, EAA-30%; gray circle, StD. ANOVA and Bonferroni t-test, * p < 0.05 vs. all diets.