Table 2.
Body weight (BW), body length (BL) and organ weight after 2, 12 and 18 months (mean ± sd).
| NEAA-100% | EAA-30% | EAA-100% | StD | Casein-Prot | Casein-AA | |||
|---|---|---|---|---|---|---|---|---|
| 2 months | F | p | ||||||
| Body W. (g) | 7.09 ± 0.41 *^ | 7.31 ± 0.52 *^ | 14.93 ± 0.62 * | 22.35 ± 1.9 | 22.02 ± 1.2 | 21.84 ± 0.9 | 238.26 | 0.000 |
| Body L. (cm) | 6.61 ± 0.1 *^ | 6.63 ± 0.12 *^ | 9.15 ± 0.13 * | 9.66 ± 0.1 | 9.62 ± 0.2 | 9.47 ± 0.17 | 1024.88 | 0.000 |
| Heart (g) | 0.08 ± 0.015* | 0.08 ± 0.01* | 0.08 ± 0.03 * | 0.13 ± 0.006 | - | - | 9.75 | 0.000 |
| Kidneys (g) | 0.12 ± 0.008*^ | 0.12 ± 0.009*^ | 0.24 ± 0.028 * | 0.40 ± 0.05 | - | - | 105.71 | 0.000 |
| Liver (g) | 0.32 ± 0.049*^ | 0.34 ± 0.61*^ | 0.75 ± 0.066 * | 1.10 ± 0.17 | - | - | 70.95 | 0.000 |
| Spleen (g) | 0.02 ± 0.004 * | 0.02 ± 0.003 * | 0.05 ± 0.008 * | 0.11 ± 0.032 | - | - | 32.35 | 0.000 |
| rpWAT (g) | 0 * | 0 * | 0.02 ± 0.002 * | 0.11 ± 0.02 | - | - | 123.97 | 0.000 |
| BAT (g) | 0.02 ± 0.01*^ | 0.02 ± 0.009*^ | 0.09 ± 0.01 * | 0.13 ± 0.015 | - | - | 112.59 | 0.000 |
| Triceps (g) | 0.07 ± 0.01*^ | 0.06 ± 0.002*^ | 0.10 ± 0.011 * | 0.19 ± 0.01 | - | - | 215.38 | 0.000 |
| 12 months | F | p | ||||||
| Body W. (g) | - | - | 24.51 ± 1.9 * | 31.78 ± 1.69 | 30.39 ± 2.79 ^ | 29.56 ± 1.0 ^ | 12.95 | 0.000 |
| Body L. (cm) | - | - | 9.82 ± 0.12 | 10.0 ± 0.13 | 10.01 ± 0.14 | 9.87 ± 0.13 | 2.65 | 0.085 |
| Heart (g) | - | - | 0.15 ± 0.02 *° | 0.23 ± 0.03 | 0.18 ± 0.02 | 0.22 ± 0.05 | 6.51 | 0.000 |
| Kidneys (g) | - | - | 0.46 ± 0.06 *°§ | 0.8 ± 0.1 | 0.7 ± 0.05 | 0.69 ± 0.06 | 21.0 | 0.000 |
| Liver (g) | - | - | 1.36 ± 0.13 *° | 1.79 ± 0.2 | 1.56 ± 0.2 | 1.7 ± 0.2 | 5.13 | 0.011 |
| Spleen (g) | - | - | 0.12 ± 0.03 | 0.15 ± 0.06 | 0.18 ± 0.02 | 0.15 ± 0.04 | 1.85 | 0.179 |
| rpWAT (g) | - | - | 0.07 ± 0.02 *§ | 0.17 ± 0.01 | 0.16 ± 0.08 | 0.09 ± 0.03 * | 6.39 | 0.005 |
| BAT (g) | - | - | 0.13 ± 0.02 § | 0.2 ± 0.04 | 0.23 ± 0.06 | 0.17 ± 0.03 | 5.62 | 0.008 |
| Triceps (g) | - | - | 0.18 ± 0.01 *°§ | 0.26 ± 0.02 | 0.31 ± 0.02 * | 0.34 ± 0.03 * | 54.35 | 0.000 |
| 18 months | t | p | ||||||
| Body W. (g) | - | - | 23.67 ± 1.15 * | 28.31 ± 1.04 | - | - | 6.692 | 0.000 |
| Body L. (cm) | - | - | 9.79 ± 0.11 | 9.95 ± 0.13 | - | - | 2.101 | 0.069 |
| Heart (g) | - | - | 0.15 ± 0.01 * | 0.2 ± 0.01 | - | - | 7.906 | 0.000 |
| Kidneys (g) | - | - | 0.47 ± 0.04 * | 0.68 ± 0.07 | - | - | 5.824 | 0.000 |
| Liver (g) | - | - | 1.18 ± 0.09 * | 1.6 ± 0.15 | - | - | 5.369 | 0.000 |
| Spleen (g) | - | - | 0.08 ± 0.03 * | 0.13 ± 0.04 | - | - | 2.236 | 0.000 |
| rpWAT (g) | - | - | 0.07 ± 0.03 * | 0.11 ± 0.02 | - | - | 2.481 | 0.038 |
| BAT (g) | - | - | 0.12 ± 0.01 * | 0.17 ± 0.01 | - | - | 7.906 | 0.000 |
| Triceps (g) | - | - | 0.15 ± 0.01 * | 0.21 ± 0.01 | - | - | 9.487 | 0.000 |
| 22 months | t | p | ||||||
| Body W. (g) | - | - | 22.2 ± 1.56 * | 25.01 ± 1.2 | - | - | 4.515 | 0.000 |
| Body L. (cm) | - | - | 9.75 ± 0.12 | 9.81 ± 0.14 | - | - | 0.794 | 0.444 |
Note the similar changes in organ weight in EAA-30% and NEAA-100%-fed animals compared to StD after 2 months. Furthermore, it should be noted that the EAA-100% diet causes a slowdown in the BW and OW at all times compared to StD. ANOVA and Bonferroni t-test: * p < 0.05 vs. StD, ^ p < 0.05 vs. EAA-100%, ° p < 0.05 vs. Casein-AA; § p < 0.05 vs. Casein-Prot. WAT, white adipose tissue. BAT, brown adipose tissue.