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. 2019 Jun 15;11(6):1348. doi: 10.3390/nu11061348

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Colonic MUC-2 mRNA levels and total colonic protein synthesis. Colonic MUC-2 mRNA levels were evaluated by RT-qPCR in both Gln (A) and BCAA (B) series. The total colonic protein synthesis rate was measured with the SUnSET method in Gln (C) and BCAA (D) series with puromycin incorporation. Column bar graphs show control mice (CTRL; black bars), non-supplemented activity-based anorexia mice (ABA-C; open bars) and supplemented activity-based anorexia mice (ABA-G or ABA-B; grey bars). Values without a common letter significantly differ (p < 0.05); *: p < 0.05 vs. ABA-C using t-test. The number of mice was n = 8 for CTRL, n = 5 for ABA-C and n = 5 for ABA-G (A,C) and n = 16 for CTRL, n = 16 for ABA-C and n = 14 for ABA-B (B,D).