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. 2019 Jun 11;17(6):348. doi: 10.3390/md17060348

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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mRNA expression levels of genes involved in calcium-promoting mechanism as determined using real-time PCR. Transient receptor potential cation V6 (TRPV6) (A), Transient receptor potential cation V5 (TRPV5) (B), calcium-binding protein-D9k (CaBP-D9K) (C), plasma membrane Ca-ATPase (PMCA1b) (D). Note: control, normal group; model, low calcium group; HCaCO₃, high dosage of CaCO₃ group; HGCa, high dosage of calcium gluconate group; LCPPH + LCaCO₃, low dosage of CPPH supplemented with low dosage of CaCO₃ group; MCPPH + MCaCO₃, model dosage of CPPH supplemented with model dosage of CaCO₃ group; HCPPH + HCaCO3, high dosage of CPPH supplemented with high dosage of CaCO₃ group; LCPPH-Ca, low dosage of CPPH-Ca group; MCPPH-Ca, model dosage of CPPH-Ca group; HCPPH-Ca, high dosage of CPPH-Ca group. Data are expressed as the mean ± SD (n = 10). One-way ANOVA with Tukey’s test. Different letters indicate significant effect with p < 0.05.