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. 2019 Jun 17;20(12):2957. doi: 10.3390/ijms20122957

Figure 2.

Figure 2

Quercetin attenuates the expression of ICAM-1, sICAM-1, IL-6, IL-8 and MCP-1 in IL-1β-stimulated ARPE-19 cells. (A) Effects of quercetin on ARPE-19 cell viability. ARPE-19 cells were treated for 24 h with 2.5–40 µM quercetin and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to analyze the cell viability. (B) ICAM-1 protein level was evaluated by Western blotting and then quantified using Image Lab software. (C) The levels of sICAM-1, (D) IL-6, (E) IL-8 and (F) MCP-1 were assessed by ELISA after cells were incubated for 1 h with quercetin at the indicated doses and then activated with 1 ng/mL IL-1β for 24 h. The data are expressed as mean ± SD of three independent experiments. # p < 0.05 versus control cells. * p < 0.05 versus IL-1β-stimulated cells.