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. 2019 Jul 9;11:6323–6341. doi: 10.2147/CMAR.S198911

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© 2019 Cui et al.

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TfR1 expression was downregulated by lentivirus-mediated shRNA. TfR1 mRNA expression was verified by real-time PCR, showing that TfR1 expression in Lenti-shTfR1 cells was significantly lower than in control cells in both SW480 (A) and SW620 (B) cell lines, ***P＜0.001. (C) The downregulation of TfR1 protein expression was assessed by Western blot.