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. 2019 Jun 25;8(6):636. doi: 10.3390/cells8060636

Figure 3.

Figure 3

Dexamethasone modulates SP7 (Osterix) and PPARG genes expression in hSDSCs exposed to TGF-β1 and BMP-2. Cells were cultured for 21 days in the presence of 10 ng/mL TGF-β1 (positive control) or in its absence (negative), low TGF-β1 concentration (1 ng/mL TGF-β1), various concentrations of BMP-2 alone (1 ng/mL BMP-2, 5 ng/mL BMP-2, 10 ng/mL BMP-2) or BMP-2 in combination with TGF-β1 (1 ng/mL TGF-β1 + 1 ng/mL BMP-2, 1 ng/mL TGF-β1 + 5 ng/mL BMP-2 and 1 ng/mL TGF-β1 + 10 ng/mL BMP-2). The amounts of SP7 (A) and PPARG (B) mRNA were normalized to RPLP0. The levels of gene expression of cells exposed to different media in the presence or absence of 100 nM dexamethasone were plotted as a fold change relative to the expression of the corresponding gene in undifferentiated cells (hSDSC day 0) defined as 1 (mean ± SD; n = 4). Grey bars represent scatterplot of different measurement in hSDSC exposed to dexamethasone, red bars represent scatterplot of different measurement in hSDSC not exposed to dexamethasone. Data are expressed as mean ± SD; significant difference from dexamethasone treatment in the same group is marked by red asterisks; significant difference among groups is marked by black asterisks (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001).