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SPR sensorgrams of isatin interaction with PTGIS immobilized on the SA chip using the streptavidin–biotin coupling protocol. Biosensor signals represent the difference between working and control channels. About 9000 RU (approximately 9 ng) of biotinylated PTGIS was immobilized on the SA chip. The following concentration of isatin were injected for 5 min at a flow rate of 10 µL/mL: (1) −43 µM, (2) −86 µM, (3) 129 µM. HBS-EP+ buffer was used as a running buffer.
