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. 2019 May 30;11(6):758. doi: 10.3390/cancers11060758

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Characterisation of glioma associated stem cell (GASC)-derived exosomes. (A) Representative graphs of nanoparticle tracking analysis (NTA) on a preparation obtained from a nonconditioned supernatant (Ctrl) and on exosomes (Exo) isolated from supernatants conditioned for 48 h by 4 GASC cell populations (Exo#1, Exo#2, Exo#3 and Exo#4). (B) Western Blotting for exosome-specific markers (tetraspanins CD63, CD9 and TSG-101) and for cell endoplasmic reticulum marker (calnexin) on 4 exosomes lysates (Exo#1–4) and on paired producing cells (GASC#1–4).