Figure 6.

(A) RES generated ROS biphasically modulates PKC activity. HUVECs were grown in M199 medium containing 5% FCS and then treated for 40 min with the indicated concentrations of RES and then PKC activity was measured as described in the materials and methods section. (B) Same as in A, but in the absence or presence of Tempol. HUVECs were pre-treated or not for 1 hr with the ROS scavenger Tempol (100 µM). PKC activities are expressed as % of control (ctrl). (+): positive control of PKC activation (phosphatidylserine 0.2 mg/mL). Means ± SDs of a representative experiment, performed in triplicates, are shown. *, significantly different from the control (p < 0.01).