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. 2019 Jun 9;8(6):562. doi: 10.3390/cells8060562

Figure 6.

Figure 6

Lack of TTYH1 or TTYH2 alone in HepG2 or LoVo cells almost completely eliminates VRAC activity. (a) Efficiency of gene silencing by the TTYH1 shRNA construct was monitored by RT-PCR in HepG2 cells. (b) Representative traces of VRAC currents from HepG2 cells transfected either with scrambled or TTYH1 shRNA in isotonic and hypotonic solutions. (c) Summary bar graph showing current densities of transfected HepG2 cells in isotonic and hypotonic solutions, as in (b), at +100 mV. (d) Efficiency of gene silencing by the TTYH2 shRNA construct was monitored by RT-PCR. (e) Representative traces of VRAC currents from LoVo cells transfected with scrambled or TTYH2 shRNA in isotonic and hypotonic solutions. (f) Summary bar graph showing current densities of transfected LoVo cells in isotonic and hypotonic solutions, as in (e), at +100 mV. Data are presented as means ± SEM (** P < 0.01, **** P < 0.0001).