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. 2019 Jun 15;8(6):593. doi: 10.3390/cells8060593

Table 1.

Kinetic parameters of Na-K-ATPase transporters in IEC-18 cells.

0 Day 1 Day 2 Day 3 Day 4 Day
Vmax
(picomole/mg protein·30 S)
742.5 ± 62 714 ± 83.5 718 ± 58.4 713 ± 27.9 733 ± 44.8
Km
(mM)
0.68 ± 0.11a 0.61 ± 0.15a 0.39 ± 0.08b 0.37 ± 0.04b 0.33 ± 0.06b

Uptake of 86Rb+ as a function of varying concentration of extracellular Rb+ (RbCl). As extracellular Rb+ concentration was increased, uptake of 86Rb+ was stimulated and subsequently became saturated in all groups. Kinetic parameters [maximal uptake (Vmax) and affinity (1/Km)] were obtained after the analysis of data with GraphPad Prism 7 software (GraphPad Software Inc., San Diego, CA, USA). Values are represented as mean ± SEM, n = 6. Values not sharing common superscripted letters are significantly different at p < 0.01.