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. 2019 Jul 10;11:6457–6466. doi: 10.2147/CMAR.S200234

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© 2019 Han et al.

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(A) Heatmap of differentially expressed genes between osteosarcoma tissues and tissues adjacent to cancer. (B and C) Expression levels of FGF5 in osteosarcoma cell lines and tissues detected by qRT-PCR, the results showed that FGF5 mRNA expression was significantly increased in osteosarcoma cell lines and tissues. (D) ICH staining for FGF5 in osteosarcoma and matched normal tissues. (E and F) Protein expression levels of FGF5 in osteosarcoma cell lines and tissues detected by Western blot, the results showed that FGF5 protein expression was upregulated in osteosarcoma cell lines and tissues. Error bars indicated mean ± SD (*P<0.01).

Abbreviations: HOB, human normal osteoblast; FGF5, fibroblast growth factor-5; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.