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. 2019 Jul 10;11:6457–6466. doi: 10.2147/CMAR.S200234

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© 2019 Han et al.

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(A) The expression of FGF5 in two osteosarcoma cell lines after FGF5 knockout by Crispr/Cas9, the results showed that FGF5 was dramatically decreased in FGF5-KO MG63 an U20S cells. (B and C) Effects of FGF5 on cell proliferation and colony formation assessed by CCK-8 and colony formation assays. The results showed that knockout of FGF5 inhibited cell proliferation. (D) Effects of FGF5 on tumor growth determined by nude mouse orthotopic metastatic model. (E) ICH staining for FGF5 in nude mice. Error bars indicated mean ± SD (*P<0.05, **P<0.01).

Abbreviations: Crl, control; KO, knockout; FGF5, fibroblast growth factor-5; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.