Figure 6.

Localizations of Lgr5⁺ stem cells and pMET in the corpus epithelium of the adult stomach. (A) Immunohistochemical staining for GFP and pMET. (B) Double immunofluorescence staining for GFP and pMET. In (A), black arrows, Lgr5-driven GFP; red arrowheads, pMET in the glandular base region; yellow arrowheads, pMET in the isthmus and/or surface regions. In (B), white arrows indicate the co-localization of pMET and GFP in Lgr5⁺ stem cells. The images in the lower panel are magnified images of the boxed areas in the upper panel. Tissues were obtained from Lgr5-DTR-EGFP mice. Scale bars represent 200 µm. (C) The numbers of GFP- and pMET-positive cells per corpus gland. These values were obtained by counting in 10 glands (n = 10). Data were tested for significance using an unpaired two-tailed t-test. NS, not significant.