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Immunoblotting of caspase-3, caspase-9 and Bcl-2. BEAS2B cells were exposed to 10% cigarette smoke extract (CSE) and/or ultrafine carbon particle (UFCP) for 24 hrs. Cells were harvested with cell lysis buffer and subjected for immunoblotting as described in the methods. (A) Representative image of the immunoblotting. (B) Semi-quantification of caspase-3. *p<0.05 compared to control. (C) Semi-quantification of caspase-9. (D) Semi-quantification of Bcl-2. *p<0.05.
