. 2019 Jun 15;10(6):459. doi: 10.3390/genes10060459

Table 1.

The primers used for PAH mutants preparation using site-directed mutagenesis. Bolded nucleotide represents the substituted base.

PAH Mutant		Primer Sequence (5′→3′)
p.Asn167Tyr	c.499A > T	CATTGCCTACTACTACCGCC
p.Asn167Tyr	c.499A > T	TCAGCAAACTGCTTCCGTC
p.Thr200Asn	c.599C > A	CTTGTATAAAAACCATGCTTGC
p.Thr200Asn	c.599C > A	GACTTCAGAGTCTTGAACACTGT
p.Asp229Gly	c.686A > G	CAGCTGGAAGGCGTTTCTCA
p.Asp229Gly	c.686A > G	GGGAATGTTATCTTCATGGAAGCC
p.Gly239Ala	c.716G > C	ACTTGCACTGCTTTCCGCCT
p.Gly239Ala	c.716G > C	CTGCAGGAACTGAGAAACGTCTTCC
p.Phe263Ser	c.788T > C	TTCCGAGTCTCCCACTGCACA
p.Phe263Ser	c.788T > C	GGCCAGGCCACCCAAGAAAT
p.Ala342Pro	c.1024G > C	CTCCATAAAGCCATATGGTGCTG
p.Ala342Pro	c.1024G > C	TCTCCTTGTTTGCAGAGCCC
p.Ile406Met	c.1218A > G	TGCCACAATGCCTCGGCCCT
p.Ile406Met	c.1218A > G	GCAAAGTTCCTTACTTTCTCCTTGGCATCATT