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Female karyotypes of Lebiasina bimaculata (a–c) and Lebiasina melanoguttata (d–f) arranged after different cytogenetic procedures. Giemsa staining (a,d), C-banding (b,e), and dual-color fluorescence in situ hybridization (FISH) with 18S (red) and 5S (green) ribosomal DNA probes (c,f). Chromosomes were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) in blue. The inserts highlight the homomorphic condition related to pair 3 in the males of L. bimaculata. Scale bar = 5 μm.
