Figure 2.

Distribution of LM5 epitope in cross sections in differentiated zone (A,A’–C,C’) and longitudinal sections in meristematic zone (A”–C”) of root tissues of barley cv. Sebastian. (A–A”)—control pH 6; (B–B”)—control pH 4; (C–C”)—30 µM AlCl₃. (A–C) insets—higher magnification of cortex cells. Detection of LM5 epitope (A–C), Calcofluor (CF) and DAPI staining with immunolocalization of LM5 epitope (A’–C’, A”–C”) are shown. (B”,C”)—arrowheads show newly arising cell walls in exodermis and cortex with presence of LM5 epitope, arrow shows the newly formed anticlinal wall with LM5 epitope absent. Epitope distribution in representative sections are shown.