Figure 3.

Distribution of LM6 epitope in cross sections in differentiated zone (A,A’–C,C’) and longitudinal sections in meristematic zone (A”–C”) of root tissues of barley cv. Sebastian. (A–A”)—control pH 6; (B–B”)—control pH 4; (C–C”)—30 µM AlCl₃. (A–C) insets—higher magnification of rhizodermal cells. Detection of LM6 epitope (A–C), Calcofluor (CF) and DAPI staining with immunolocalization of LM6 epitope (A’–C’, A”–C”) are shown. (B,C)—arrowheads within insets show the presence of LM6 epitope in cytoplasmic compartments in rhizodermal cells, (B’,C’)—asterisk symbols show the xylem cells with presence of LM6 epitope, A”–C” —arrows show the remnants of root cap cells with the LM6 epitope recognized. Epitope distribution in representative sections are shown.