Figure 5.

Distribution of LM20 epitope in cross sections in differentiated zone (A,A’–C,C’) and longitudinal sections in the meristematic zone (A”–C”) of root tissues of barley cv. Sebastian. (A–A”)—control pH 6; (B–B”)—control pH 4; (C–C”)—30 µM AlCl₃. (A–C) insets—higher magnification of cortex cells with developing intercellular spaces. Detection of LM20 epitope (A–C), Calcofluor (CF), and DAPI staining with immunolocalization of LM20 epitope (A’–C’,A”–C”) are shown. (A–C)—arrowheads within insets show developing intercellular spaces with LM20 epitope recognized. C”—arrow shows the LM20 signal in the outer wall of cell from the complex arising from the cortex “mother” cell division. Epitope distribution in representative sections are shown.