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The example of the negative control for the immunolocalization of pectin epitopes in cross sections in differentiated zone of root tissues of barley cv. Sebastian. (A)—control pH 6; B—control pH 4; C—30 µM AlCl3. Calcofluor (CF) and DAPI staining (A–C), no primary antibodies in immunolocalization reaction were used (A’–C’) and merged CF and DAPI staining with negative control are shown (A”–C”). Representative sections are shown.
