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. 2019 Jun 19;11(6):853. doi: 10.3390/cancers11060853

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Myoferlin is required for PDAC cell migration and OXPHOS. BxPC-3 and Panc-1 cells were silenced for myoferlin with two different small interfering RNAs (siRNAs). (A) Two-dimension migration kinetic assay (scratch assay) of BxPC-3 and Panc-1 cell lines silenced for myoferlin. (B) Representative images of 3D migration evaluated in Boyden’s chamber for 24 h. (C) Quantification of migrating BxPC-3 and Panc-1 depleted for myoferlin in the lower compartment of Boyden’s chamber. (D) Kinetic oxygen consumption rate (OCR) response of irrelevant or myoferlin siRNA-transfected BxPC-3 and Panc-1 cells to oligomycin (oligo, 1 µM), FCCP (1.0 µM), and rotenone and antimycin A mix (Rot/Ant, 0.5 µM each). Upon assay completion, cells were methanol/acetone fixed, and cell number was evaluated using Hoechst incorporation (arbitrary unit, A.U.). One representative experiment out of three is illustrated. Same results were obtained with the second myoferlin siRNA. Each data point represents mean ± SD, n = 3. **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05.