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. 2019 Jun 19;11(6):853. doi: 10.3390/cancers11060853

Figure 5.

Figure 5

Myoferlin increases OXPHOS activity in HM clones. (A) Kinetic oxygen consumption rate (OCR) response of Panc-1 clones (LM1-3 and HM1-3) to oligomycin (oligo, 1 µM), FCCP (1.0 µM), and rotenone and antimycin A mix (Rot/Ant, 0.5 µM each). Upon assay completion, cells were methanol/acetone fixed, and cell number was evaluated using Hoechst incorporation (arbitrary unit, A.U.). (B) Metabolism and glutamine-related enzyme gene expression analysis by RT-PCR in LM3 and HM3 Panc-1 clones. (C) Cytochrome c oxidase (COX IV) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1-α abundance in LM and HM clones. HSC70 was used as a loading control. (D) Kinetic oxygen consumption rate (OCR) response of Panc-1 clones (HM3) silenced for myoferlin to oligomycin (oligo, 1 µM), FCCP (1.0 µM), and rotenone and antimycin A mix (Rot/Ant, 0.5 µM each). Upon assay completion, cells were methanol/acetone fixed, and cell number was evaluated using Hoechst incorporation (arbitrary unit, A.U.). (E) Two-dimension migration kinetic assay (scratch assay during 16 h) of HM3 Panc-1 clone silenced for myoferlin. One representative experiment out of three is illustrated. Each data point represents mean ± SD, n = 3. **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05.