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SDS-PAGE analysis of WT PlyC CHAP and its mutants. The solubility and purity of each enzyme after the His-tag cleavage were assessed via a 7.5% SDS-PAGE gel. The lanes correlate to: (M) BioRad protein markers; (1) PlyC CHAP WT; (2) PlyC CHAP +1; (3) PlyC CHAP +2; (4) PlyC CHAP +3; (5) PlyC CHAP +4; (6) PlyC CHAP +5; (7) PlyC CHAP +6, and; (8) PlyC CHAP +7.
