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In vitro lytic activity against S. pyogenes D471. The different concentrations of enzymes were added to the overnight bacterial cultures. The OD600 was recorded every 15 seconds for 1 hour. The OD600 decrease, which is the net change in OD600 between the PBS treated control and enzyme treatment for 1 hour, was represented as the enzyme activity. The experiment was conducted in triplicate, and the error bars represent the standard deviation.
