Figure 1.

Overview of the experimental setup. Mesenchymal stromal cells (MSCs) of 10 patients were harvested and isolated. Next, the cells were expanded to passage 3 in cell culture flasks, and then seeded on 24 well plates. Ten replicates for the individual setting, consisting of 2 technical replicates each, and 10 replicates for the pooled setting. After designated incubation times, Alizarin Red S staining (ALZ), alkaline phosphatase activity measurement (ALP) and fluorescein diacetate (FDA)/propidium iodide (PI) staining (FDA/PI) were performed.